If an unknown glucose solution reading exceeds the calibration curve, what is one option to obtain an accurate concentration?

Creating a dilution series with the unknown sample is indeed an effective method to obtain an accurate concentration when the reading exceeds the calibration curve. When a solution's concentration surpasses the range established by the calibration curve, it indicates that the absorbance (or measurement) may be outside the linear portion of the curve. In this scenario, the readings can become unreliable or inaccurate.

By diluting the unknown sample, the concentration is reduced to a level that falls within the established calibration curve. This allows for the use of the linear relationship between concentration and absorbance again, providing an accurate determination of the original concentration based on the diluted measurement. After measuring the absorbance of the diluted samples, one can then apply the dilution factor to calculate the concentration of the original solution confidently.

Relying solely on the higher reading from the colorimeter could lead to erroneous conclusions about the concentration, ignoring the potential inaccuracies caused by being outside the calibration range. Ignoring the readings entirely and assuming a standard concentration disregards the actual measurements and variations that may exist in the sample. Using a weaker colorimetric analysis method may compromise accuracy and sensitivity, ultimately failing to resolve the initial issue of exceeding the calibration range. Therefore, creating a dilution series provides a systematic and reliable approach to achieve