What is the best technique for cutting sections of stem as thin as possible?

Cutting sections of stem very close to the end is crucial for obtaining the thinnest and most precise slices. This technique ensures that the cuts are made at the point where the plant tissue is most pliable and delicate, thereby allowing for thinner sections compared to cutting further away from the end. Additionally, cutting close to the end minimizes the amount of material that may become compressed or distorted during the slicing process, which can happen if the cuts are made further along the stem. This results in more uniform, thin sections that are critical for various applications, including microscopy or other forms of analysis where the quality of the section can greatly impact results.

Using a straight edge for cuts may improve precision but does not necessarily lead to thinner sections. Cutting at an angle could provide a better surface area for exposure but can compromise the thickness of the slices. Slicing in bulk, while it may save time, typically leads to uneven thickness and lower quality of sections. Thus, cutting close to the end of the stem directly addresses the need for thin, uniform samples.